Mohammad Forouhar Vajargah; Hamed Ghafari Farsani; Saeid Shahbazi Naserabad; Aliakbar Hedayati
Abstract
Abstract Different organisms like aquatics are constantly exposed to pesticide residues, this study's overall assessment patho-biological changes in gills and livers of Capoeta capoeta gracilis during exposure to butachlor pollutant. First of all the 96-hour lethal concentration of the toxin was calculated ...
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Abstract Different organisms like aquatics are constantly exposed to pesticide residues, this study's overall assessment patho-biological changes in gills and livers of Capoeta capoeta gracilis during exposure to butachlor pollutant. First of all the 96-hour lethal concentration of the toxin was calculated as 46.2 mg per liter. Then, 96 fish were divided into four groups: one control group and three experimental groups (with concentrations of 0.2, 0.4, and 0.8 mg l-1) and were exposed for 2 weeks. In the end point, histological changes were studied by hematoxylin and eosin staining method in light microscopy. Lethal concentration of this toxin was 2.46 mg/l that means moderate toxicity of this species. There was many histological changes in the gills of fish treated with butachlor include: hyperplasia, shortening the secondary lamella, edema, fusion, hemorrhage and atrophy in compared to the control group. Treatment with liver butachlor cause obstruction, dark granules, necrosis, and bile stagnation and sinusoid dilution in studied fish. The results of this study showed that in both studied organs with increase of toxin concentration, more severe tissue damage was observed, so gill and liver tissues of this fish could be used as an appropriate biomarker for pollutant measurement.
Mohammad Forouhar Vajargah; Seyed Aliakbar Hedayati
Abstract
Abstract One of the most important factors that today is important with the risks associated of the growing industry and technology, is plant pesticides that can lead to the destruction of aquatic communities in the long times by entering aquatic ecosystems. So in current study acute toxicity of butachlor ...
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Abstract One of the most important factors that today is important with the risks associated of the growing industry and technology, is plant pesticides that can lead to the destruction of aquatic communities in the long times by entering aquatic ecosystems. So in current study acute toxicity of butachlor poison that is a very common at the agricultural plant was studied in 5-7 gr common carp and Caspian roach for detection of LC50 at 96 hour. Experiment was in static plan and based on the OECD standard conducted for 4 days; water physicochemical parameters including PH, dissolved oxygen and temperature were measured that they were in suitable ranges. Based on the results, acute toxicity of butachlor on carp and Caspian roach were 0.785 and 0.258 mg/l respectively and maximum allowable concentration MAC value were calculated as 0.078 and 0.025 mg/l respectively. According to the standard for determine of the toxicity of various pesticides, butachlor was considered more highly toxic for Caspian roach than common carp.
Aliakbar Hedayati; Abdolreza Jahanbakhshi; Mohammad Moradzadeh
Volume 4, Issue 1 , September 2015, , Pages 27-34
Abstract
Abstract In respect to nano-technological studies and physiological and biochemical lesions of these materials on aquatic animals, in this study we examine the toxicity effects of nano-zinc oxide (ZnO NPs) on hematological indices of common carp (Cyprinus carpio). LC50 of nano zinc oxide was determined ...
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Abstract In respect to nano-technological studies and physiological and biochemical lesions of these materials on aquatic animals, in this study we examine the toxicity effects of nano-zinc oxide (ZnO NPs) on hematological indices of common carp (Cyprinus carpio). LC50 of nano zinc oxide was determined according to the mortality at 24, 48, 72 and 96 hours, respectively. During separate experiment based on the LC50 levels, hematological parameters were studied with induction of the sub-lethal concentrations of nano zinc oxide. In this study, lethal toxicity concentration of nano-ZnO (LC50) for common carp was 3.120 ± 0.23 ppm. when the fish were exposed to sub lethal concentrations (50% of LC50) for 7days, the results showed that the nano-particles causes various changes in the blood parameters of the fish that these changes was affected by the nano-particles in blood indices by reducing the level of red blood cells (RBC), Hemoglobin, Hematocrit and increase in white blood cells (WBC) (P0.05) in the exposed nano-ZnO. The results of this study showed that the sub-lethal toxicity of nano-particles could have a negative impact on hematological indices of Common carp, so we should prevent the entrance of such substances to the aquatic ecosystems.
H. Ghafari Farsani; S. A. Akbar Hedayati; M. Mansouri Chorehi; N. Rostamian; E. Chaharde Baladehi
Volume 3, Issue 4 , July 2015, , Pages 9-16
Abstract
Abstract The aim of current study was to evaluate the effects of heavy metal cadmium on liver tissue of gold fish during experimental condition. Study was in a week on 5 treatments and 3 replicates, five concentration (2.8, 5.6, 8.4 and 11.2 ppm respectively) of cadmium as 0 (control), 25%, 50%, 75% ...
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Abstract The aim of current study was to evaluate the effects of heavy metal cadmium on liver tissue of gold fish during experimental condition. Study was in a week on 5 treatments and 3 replicates, five concentration (2.8, 5.6, 8.4 and 11.2 ppm respectively) of cadmium as 0 (control), 25%, 50%, 75% and 100% of LC50 were selected and tested. Histopathological changes were done using classic histology method in hematoxylin and eosin staining and using a light microscope and camera images properly installed and connected the camera to the computer system software was developed. The overall result of this study showed that in liver tissue exposed to cadmium over a week with increasing duration of exposure to pesticide exposure, a high level of toxin in the lesion by the most frequent lesions end of a week, was observed including necrosis, atrophy and cloudy swelling of the cell. Result of this study show that liver tissue have many lesions in response to cadmium in one week and it will be increase with concentration and duration of toxin, as it was seen highest amount of lesions at the end of experiment within last dose.
